Christoph A. Gasser, Gregor Hommes, Andreas Schäffer, Philippe F.-X. The pathway extends from 4-hydroxybenzoic acid (4-HBA), and needs only three heterologous enzymes, p-hydroxybenzoate hydroxylase (PobA), carboxylic acid reductase (CAR) and caffeate O-methyltransferase (COMT). Microbial Ligninolysis: Toward a Bottom-Up Approach for Lignin Upgrading. Vincenzo Augugliaro, Giovanni Camera-Roda, Vittorio Loddo, Giovanni Palmisano, Leonardo Palmisano, Francesco Parrino, Maria Angela Puma. zu 2-Methoxy-4-methylphenol. The solid that crystallizes (vanillyl alcohol) is collected by vacuum filtration, washed with water and allowed to air dry. Ying Zhang, Liangkun Long, Shaojun Ding. It is a member of guaiacols and a member of benzyl alcohols. Additionally, the present study demonstrates the application of enzyme promiscuity of COMT in the design of an artificial pathway for the production of high-value methylated aromatic compounds. Information. The substrate tolerance of alcohol oxidases. Jianfeng Jin, Hortense Mazon, Robert H. H. van den Heuvel, Dick B. Janssen, Marco W. Fraaije. María del Carmen Martínez-Cuesta, John Payne, Sean B. Hanniffy, Michael J. Gasson, Arjan Narbad. Jörg Overhage, Alexander Steinbüchel, Horst Priefert. Biodegradation of capsaicin by Bacillus licheniformis SK1230. Please note: If you switch to a different device, you may be asked to login again with only your ACS ID. Production of biovanillin from wheat bran. Jia Wang, Xiaolin Shen, Jian Wang, Yaping Yang, Qipeng Yuan. Synth. Harnessing eugenol as a substrate for production of aromatic compounds with recombinant strains of Amycolatopsis sp. Pranab Goswami, Soma Sekhar R. Chinnadayyala, Mitun Chakraborty, Adepu Kiran Kumar, Ankana Kakoti. Daphna Havkin-Frenkel, Faith C. Belanger. Electrochemical conversion of corn stover lignin to biomass-based chemicals between Cu/Ni Mo Co cathode and Pb/PbO 2 anode in alkali solution. Get article recommendations from ACS based on references in your Mendeley library. A. Paula Dionísio, G. Molina, D. Souza de Carvalho, R. dos Santos, J.L. Users are Pinacol coupling of aromatic aldehydes and ketones using magnesium in aqueous ammonium chloride under ultrasound. 52:2741-2746. These metrics are regularly updated to reflect usage leading up to the last few days. Files available from the ACS website may be downloaded for personal use only. Frank Hollmann, Isabel W. C. E. Arends, Katja Buehler, Anett Schallmey, Bruno Bühler. Vanillyl alcohol is a phenolic alcohol and is used as a flavoring agent in foods and beverages. M. Panouillé, M.-C. Ralet, E. Bonnin, J.-F. Thibault. Box 1, 2600 MA Delft, The Netherlands. The flavoprotein vanillyl alcohol oxidase (VAO) acts on a wide range of phenolic compounds and converts both creosol and vanillylamine to vanillin with high yield. A Xylenol Orange-Based Screening Assay for the Substrate Specificity of Flavin-Dependent para-Phenol Oxidases. Reaction of vanilmandelic acid and 4-hydroxybenzyl alcohol derivatives with L-ascorbic acid. Nicholas J. Walton, Melinda J. Mayer, Arjan Narbad. Due to increasing interest in natural vanillin, two enzymatic routes for the synthesis of vanillin were developed. The VAO-catalyzed conversion of vanillylamine proceeds efficiently at alkaline pH values. Ultrasound-assisted oxidation of vanillyl alcohol 0.2 g (1.3 mmol) of vanillyl alcohol were dissolved in distilled water at 75 °C. 31:3037-3040. Meanwhile, 499.36 mg/L vanillyl alcohol was produced by COMT in vivo catalysis when fed with 1000 mg/L 3,4-dihydroxybenzyl alcohol. Yufen Chen, Peiling Wu, Liang-Yu Ko, Tzu-Yu Kao, Lijun Liu, Yang Zhang. Recovery of Vanillin and Syringaldehyde from Lignin Oxidation: A Review of Separation and Purification Processes. Electronic Supporting Information files are available without a subscription to ACS Web Editions. Eine katalytische Hydrierung von Vanillin führt zu Vanillylalkohol bzw. Sangbeum Cho, Hyung-In Moon, Go-Eun Hong, Chi-Ho Lee, Jeong-Mee Kim, Soo-Ki Kim. Laboratory-evolved Vanillyl-alcohol Oxidase Produces Natural Vanillin. CipA-mediating enzyme self-assembly to enhance the biosynthesis of pyrogallol in Escherichia coli. Heterologous synthesis of 4-ethylphenol in engineered Escherichia coli. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States. Expanding the repertoire of aromatic chemicals by microbial production. Ghahremani, M.R. The VAO-mediated conversion of creosol proceeds via a two-step process in which the initially formed vanillyl alcohol is further oxidized …